2017-8-11 16:0:32025-05-22 10:21:27
description
unknown
similar to ribosomal RNA large subunit methyltransferase I
locus
BSU38360
BSU_38360
geneLength
1188
1191
product
unknown
putative ribosomal RNA large subunit methyltransferase
ec
outlinks
bsu
BSU38360
BSU_38360
The protein
Catalyzed reaction/ biological activity
S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing C(5)-methylcytosine (according to Swiss-Prot)
The protein
Protein family
RlmI family (according to Swiss-Prot)
[SW|Methyltransferase superfamily] (according to UniProt)
Biological materials
Mutant
MGNA-B222 (ywbD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1221 NBRP B. subtilis, Japan]
MGNA-B222 (ywbD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1221 NBRP B. subtilis, Japan]
BKE38360 (Δ[[gene|ywbD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATGTCGTCCTCTTTC, downstream forward: _UP4_TAAATGAAAAAGCTGCCCTG
BKK38360 (Δ[[gene|ywbD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATGTCGTCCTCTTTC, downstream forward: _UP4_TAAATGAAAAAGCTGCCCTG
Biological materials
lacZ fusion
GP1614 ''amyE::p(ywbD-lacZ cat)'', constructed with pGP2150 based on [[protein|pAC5]], available in [SW| Jörg Stülke]'s lab
GP1614 ''amyE::p(ywbD-lacZ cat)'', constructed with pGP2150 based on [SW|pAC5], available in [SW| Jörg Stülke]'s lab
References
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
PUA domain (aa 1-79) (according to UniProt)
The protein
Structure
[PDB|3VSE] (from Staphylococcus aureus, 48% identity) [pubmed|23016631]